Desthiobiotinisasingle-ring,sulfur-freeanalogofbiotinthatbindstostreptavidinwithnearlyequalspecificitybutlessaffinitythanbiotin(Kd=1011vs.1015M,respectively).Consequently,desthiobiotinylatedproteinscanbeelutedreADIlyandspecificallyfromstreptavidinaffinityresinusingmildconditionsbasedoncompetitivedisplacementwithfreebiotin.Forpull-downexperimentswithBIOLOGicalsamples,thissoft-releasecharacteristicofdesthiobiotinalsohelpstominimizeco-purificationofendogenousbiotinylatedmolecules,whichremainboundtostreptavidinuponelutionofthetargetproteincomplexeswithfreebiotin.
SelectedReferences:
(a)Hirsch,J.D.,atal.(2002).Anal.Biochem.,308:343-357.(b)Hofmann,K.,etal.(1982)Biochemistry,21:978-984.(c)Romovacek,H.,etal.(1983)Biochemistry,22:904-909.(d)Finn,F.M.,etal.(1984)Biochemistry,23:2554-2558.(e)Busse,S.,etal.(2002)BiosensorsandBioelectronics,17:704-710.(f)Yoon,H.C.,etal.Langmuir,171234-1239.
| Molecularweight | 311.34 |
| Chemicalcomposition | C14H21N3O5 |
| CAS | 80750-24-9 |
| Solubility | DMSO,DMF |
| Purity | >95% |
| Appearance | Whitesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
