Click-&-GoTMProteinEnrichmentKitisanefficient,biotin-streptavidinfreetoolforcaptureofazido-modifiedproteinsonanalkyne-agaroseresin.Theazidemodificationcanoccurviametabolicfeeding,enzymaticaddition,orchemicalmodification.Theazido-modifiedproteins,ortheirpost-translationallymodifiedforms,arecapturedfromcomplexproteinextractsonthealkyne-agaroseresinsupplied.OncecovalentlyattachedtotheresinviacoppercatalyzedClickchemistry,beadscanbewashedwithhigheststringencyvirtuallyeliminatinganynon-specificallyboundproteinstoyieldahighlyenrichedpopulationofnascentmolecules.Uponproteasedigestion,thisyieldsahighlypurepeptidepoolthatisidealformassspectrometry(e.g.,LCMS/MS)basedanalysis.
Thebiotin-freeenrichmentimprovessignaltonoisebyeliminatingnonspecificbindingandincreasingselectivity,thusimprovingdetectionoflowabundantproteins.ThisapproachisfullycompatIBLewithwidelyusedMStechniquesincludingiTRACandICAT.
Thebiotin-free,covalentcaptureapproachissuperiortobiotinorlectinbasedenrichmentapproaches,andidealforproteomics,bioMarkerdiscovery,posttranslationalmodification(PTM)analysis,andmore.
TheClick-&-GoTMProteinEnrichmentKitcanbeusedasdirectreplacementofClick-iT®ProteinEnrichmentKit
| Enrichmenttarget | Azide-modifiedproteins |
| Format | SpinColumn |
| Isolationtechnology | Biotin-freecovalentcapture |
| StorageCondition | 4C |
| Shippingconditions | Ambienttemperature |
