Cy®3isabright,water-soluble,andpHinsensitiveorange-fluorescentdyethatcanbeexcitedusingthe532nmor555nmlaserlineandvisualizedwithTRITC(tetramethylrhodamine)filtersets.Cy3conjugatesgivelessbackgroundthanTAMRAandmostothercommonlyusedfluorescentdyes.
Cy®3azidecanbereactedwithterminalalkynesviaacopper-catalyzedclickreaction(CuAAC).Italsoreactswithstrainedcyclooctyneviaacopper-free“Clickchemistry”reactiontoformastabletriazoleanddoesnotrequireCu-catalystorelevatedtemperatures.Cy3isawater-soluble,brightfluorescentlabelthatcanbeusedinplaceofAlexaFluor555®,DyLight549®andotherdyesexcitableatawavelengthof532or555nm.
Forthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesiredpleaseconsiderusingnextgenerationazidesprobescontaininganinternalcopper-chelatingmotif.
Learmmoreaboutnextgenerationazidesprobes
Thissulfonateddyeisalsoknownassulfo-Cyanine3Azide.Cy®DyeisatrademarkofGEHealthcare.
| Abs/EmMaxima | 550/570nm |
| Extinctioncoefficient | 150,000 |
| Flowcytometrylaserline | 532or555nm |
| Microscopylaserline | 532or555nm |
| Spectrallysimilardyes | AlexaFluor®546,Atto™543,CF™555Dye,DyLight™549 |
| Molecularweight | 850.92 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF,MeOH |
| Purity | >90%(HPLC) |
| Appearance | Redsolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
