5-TAMRAAzide(alsoknownasTAMRAazide,isomer5)isthered-fluorescentprobethatiscompatIBLewithvariousexcitationsourcesincludingmercuryarc,tungstenandxenonarclamps,the544nmlineoftheHelium-Neonlaserandthe532nmgreenlaserline.Itispredominantlyusedfordetectionofterminalalkyne-taggedbiomoleculesviaacopper-catalyzedclickreaction(CUAAC).Italsoreactswithstrainedcyclooctyneviaacopper-free“Clickchemistry”reactiontoformastabletriazoleanddoesnotrequireCu-catalystorelevatedtemperatures.
Althoughthemixedisomersof5(6)-TAMRAazideisapreferred,routinelyusedredfluorescentdyeforstainingproteins,labelingpeptidesandnucleotideswithTAMRAmixedisomersmightbetroublesomeduetosignificantsignalbroadeninginHPLCpurification.PeptidesandnucleotideslabeledwithasingleisomerTAMRAusuallygivebetterresolutioninHPLCpurificationthatisoftenrequiredintheconjugationprocesses.
5-TAMRAazideisexactreplacementofInvitrogen’s(ThermoFisherScientific)Tetramethylrhodamine(TAMRA)Azide(Tetramethylrhodamine5-Carboxamido-(6-Azidohexanyl)),5-isomer(Catalognumber:T10182).
| Abs/EmMaxima | 553/575 |
| Extinctioncoefficient | 92,000 |
| Flowcytometrylaserline | 532or555nm |
| Microscopylaserline | 532or555nm |
| Spectrallysimilardyes | AlexaFluor®546,Atto™543,CF™555Dye,DyLight™549 |
| Molecularweight | 555.67 |
| CAS | 1006592-61-5 |
| Solubility | Water,DMSO,DMF,MeOH |
| Purity | >98%(HPLC) |
| Appearance | Redsolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
