OG488Azide,(alsoknownasOregonGreen®488orfluorinatedcarboxyfluorescein)isabright,green-fluorescentprobethatcanbereactedwithterminalalkynesviacopper-catalizedclickreactionorstraincyclooctynes
Withexcitationideallysuitedtothe488nmlaserline.ThefluorinatedcarboxyfluoresceinshavehigherphotostABIlityandionizeatalowerpH(pKa4.8)thanfluorescein(pKa)6.5),whichmakesthemsuperiorfluorescentdyesforuseasreportermoleculesinBIOLOGicalsystems.
Inaddition,theabsorptionandemissionofOG-488dyes(alsosoldunderOregonGreen®488tradename)arevirtuallyidenticaltowidelyusedfluoresceinsdyes.
Thecombinationofhigherphotostability,lowerpKa,andexcitationideallysuitedtothe488nmlaserlinemakesOG-488dyesoneofthebestreplacementsforthewidelyusedfluoresceindyes.
OG488azideisexactreplacementofInvitrogen’s(ThermoFisherScientific)OregonGreen®488Azide(OregonGreen®6-Carboxamido-(6-Azidohexanyl),TriethylammoniumSalt),6-isomer(Catalognumber:O10180).
| Abs/EmMaxima | 496/524nm |
| Extinctioncoefficient | 84,200 |
| Flowcytometrylaserline | 488nm |
| Microscopylaserline | 488nm |
| Spectrallysimilardyes | FAM,AlexaFluor®488,Atto™488,CF™488Adyes,DyLight™488, |
| Molecularweight | 536.49 |
| CAS | N/A |
| Solubility | DMSO,DMF |
| Purity | >95% |
| Appearance | Yellowtoorangesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
