Cy5.5PicolylAzideisanadvancedfluorescentprobethatincorporatesacopper-chelatingmotiftoraisetheeffectiveconcentrationofCu(I)atthereactionsite.Thechelatingeffectofpicolylazidemoietytoraisetheeffectiveconcentrationofcopperatthereactionsiteissogreatthatitleadstoanincreaseofsignalintensityofupto40-fold.
Inaddition,theuseofCy5.5PicolylAzideinsteadofconventionalCy5.5-Azideallowsforatleastatenfoldreductionintheconcentrationofthecoppercatalystwithoutsacrificingtheefficiencyoflabeling.
Insummary,theintroductionofapicolylmoietyintoanazideprobeleadstoasubstantialincreaseinthesensitivityofalkynedetection.Thiswillbeofspecialvalueforthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesired.
Learnmoreaboutpicolylazidereagents.
Thissulfonateddyeisalsoknownassulfo-Cyanine5.5Azide.Cy®DyeisatrademarkofGEHealthcare.
| Abs/EmMaxima | 678/694nm |
| Extinctioncoefficient | 190,000 |
| Spectrallysimilardyes | AlexaFluor®680,IRDye®680RD,CF™680Dye,DyLight™680 |
| Molecularweight | 1119.26 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF |
| Purity | >95%(HPLC) |
| Appearance | Bluesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
