5-TAMRAPicolylAzideisanadvancedfluorescentprobethatincorporatesacopper-chelatingmotiftoraisetheeffectiveconcentrationofCu(I)atthereactionsite.Thechelatingeffectofpicolylazidemoietytoraisetheeffectiveconcentrationofcopperatthereactionsiteissogreatthatitleadstoanincreaseofsignalintensityofupto40-fold.Inaddition,theuseof5-TAMRAPicolylAzideinsteadofconventional5-TAMRAAzideallowsforatleastatenfoldreductionintheconcentrationofthecoppercatalystwithoutsacrificingtheefficiencyoflabeling.
Althoughthemixedisomersof5(6)-TAMRAisapreferred,routinelyusedredfluorescentdyeforstainingproteins,labelingpeptidesandnucleotideswithTAMRAmixedisomersmightbetroublesomeduetosignificantsignalbroadeninginHPLCpurification.PeptidesandnucleotideslabeledwithasingleisomerTAMRAusuallygivebetterresolutioninHPLCpurificationthatisoftenrequiredintheconjugationprocesses.
Insummary,theintroductionofapicolylmoietyintoanazideprobeleadstoasubstantialincreaseinthesensitivityofalkynedetection.Thiswillbeofspecialvalueforthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesired.
Learnmoreaboutpicolylazidereagents.
| Abs/EmMaxima | 545/576 |
| Extinctioncoefficient | 91,000 |
| Flowcytometrylaserline | 532or555nm |
| Microscopylaserline | 532or555nm |
| Spectrallysimilardyes | AlexaFluor®546,Atto™543,CF™555Dye,DyLight™549 |
| Molecularweight | 808.89 |
| CAS | N/A |
| Solubility | DMSO,DMF,MeOH |
| Purity | >97%(HPLC) |
| Appearance | Redsolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
