MB™594PicolylAzideisanadvancedfluorescentprobethatincorporatesacopper-chelatingmotiftoraisetheeffectiveconcentrationofCu(I)atthereactionsite.Thechelatingeffectofpicolylazidemoietytoraisetheeffectiveconcentrationofcopperatthereactionsiteissogreatthatitleadstoanincreaseofsignalintensityofupto40-fold.
Inaddition,theuseofMB™594PicolylAzideinsteadofconventionalMB™594Azideallowsforatleastatenfoldreductionintheconcentrationofthecoppercatalystwithoutsacrificingtheefficiencyoflabeling.
Insummary,theintroductionofapicolylmoietyintoanazideprobeleadstoasubstantialincreaseinthesensitivityofalkynedetection.Thiswillbeofspecialvalueforthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesired.
MB™594isabright,redfluorescentprobewithabsorptionandemissionmaximasimilartoTexasReddyeorAlexaFluor594®.Thedyeisbasedonanovelclassofrhodaminedyes.UnliketrADItionalrhodaminedyes,MB™594isnotonlybrightandphotostablebutalsohighlywater-solubleandpH-insensitive.MB™594canbeusedasareplacementforAlexaFluor594®,DyLight594®,andTexasRed.
Learnmoreaboutpicolylazidereagents.
| Abs/EmMaxima | 601/623 |
| Extinctioncoefficient | 110,000 |
| Flowcytometrylaserline | 532or594nm |
| Microscopylaserline | 594nm |
| Spectrallysimilardyes | AlexaFluor®594,Atto™594,CF™594Dye,DyLight™594 |
| Molecularweight | ca.1050 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF,MeOH |
| Purity | >95%(HPLC) |
| Appearance | Bluesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
