MB™488PicolylAzideisanadvancedfluorescentprobethatincorporatesacopper-chelatingmotiftoraisetheeffectiveconcentrationofCu(I)atthereactionsite.Thechelatingeffectofpicolylazidemoietytoraisetheeffectiveconcentrationofcopperatthereactionsiteissogreatthatitleadstoanincreaseofsignalintensityofupto40-fold.
Inaddition,theuseofMB™488PicolylAzideinsteadofconventionalMB™488Azideallowsforatleastatenfoldreductionintheconcentrationofthecoppercatalystwithoutsacrificingtheefficiencyoflabeling.
Insummary,theintroductionofapicolylmoietyintoanazideprobeleadstoasubstantialincreaseinthesensitivityofalkynedetection.Thiswillbeofspecialvalueforthedetectionoflowabundancetargetsorwheresignificantincreaseinsignalintensityisdesired.
MB™488PicolylAzideisabright,greenfluorescentprobewithexcitationideallysuitedforthe488nmlaserline.MB™488isbright,exceedinglyphotostableandpH-insensitivefluorescentdyethatcanbeusedinplaceofFluorescein,AlexaFluor488®,DyLight488®andotherdyesexcitableatawavelengthof488nm.
Learnmoreaboutpicolylazidereagents.
| Abs/EmMaxima | 495/515nm |
| Extinctioncoefficient | 75,000 |
| Flowcytometrylaserline | 488nm |
| Microscopylaserline | 488nm |
| Spectrallysimilardyes | FAM,AlexaFluor®488,Atto™488,CF™488Adyes,DyLight™488, |
| Molecularweight | ca.850 |
| CAS | N/A |
| Solubility | DMSO,DMF,MeOH |
| Purity | >95% |
| Appearance | Redtoorangesolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
