DdeBiotinPicolylAzideisauniqueaminereactive,cleavablebiotinprobethatallowsforreleaseofthecapturedbiomoleculesfromstreptavidinundermildconditions.ThisreagentcontainsabiotinmoietylinkedtopicolylazidemoetythroughaspacerarmcontainingahydrazinecleavableDdemoiety.Capturedbiomoleculescanbeefficientlyreleased,typically>90%with2%hydrazineinaqueousmedia.
DdeBiotinPicolylAzideincorporatesacopper-chelatingmotifthatdramaticallyacceleratestheCu(I)-catalyzedazide−alkynecycloaddition(CuAAC)reactionunderconditionsrelevanttobiomolecularlabeling.DiscoveryofazideswithaninternalCu(I)-chelatingmotifgreatlyenhancestheutilityofCuAACreactionformonitoringthedynamicglycanbiosynthesisinlivingorganisms,thesensitivedetectionofmetabolicallylabeledproteinsDNAsandRNAs,andformanyotherapplicationwhererateaccelerationandreducedcelltoxicityarehighlydesirable.
Learnmoreaboutpicolylazidereagents.
| Molecularweight | 815.98 |
| Molecularweightleftbehind | 220.24 |
| Chemicalcomposition | C38H57N9O9S |
| CAS | N/A |
| Solubility | DMSO,DMF,THF,DCM,Chloroform |
| Purity | >95%(HPLC) |
| Appearance | Oiltoamorphoussolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
