Methyltetrazine-activatedCy3dyethatreactswithTCO-containingcompoundsviaaInverse-Electron-DemandDiels-AlderreactiontoformastablecovalentbondanddoesnotrequireCu-catalystorelevatedtemperatures.Theinverse-electrondemandDiels-AldercycloadditionreactionofTCOwithtetrazinesisabioorthogonalreactionthatpossessesexceptionalkinetics(k>800-1s-1)andselectivity.Suchexcellentreactionrateconstantsareunparalleledbyanyotherbioorthogonalreactionpairdescribedtodate.
Cy®3isabright,water-soluble,andpHinsensitiveorange-fluorescentdyethatcanbeexcitedusingthe532nmor555nmlaserlineandvisualizedwithTRITC(tetramethylrhodamine)filtersets.Cy3conjugatesgivelessbackgroundthanTAMRAandmostothercommonlyusedfluorescentdyes.
Cy®DyeisatrademarkofGEHealthcare
Abs/EmMaxima 550/570nm Extinctioncoefficient 150,000 Flowcytometrylaserline 532555nm Microscopylaserline 532or555nm Spectrallysimilardyes AlexaFluor®555,Atto™555,CF™555Dye,DyLight™549 Molecularweight 908.07 CAS 1676067-48-3 Solubility Water,DMSO,DMF,MeOH Purity >95%(HPLC) Appearance Redsolid Storageconditions -20C Shippingconditions Ambienttemperature
