Tetrazinesdemonstrateexceptionallyfastcycloadditionkinetics(upto30000M-1s-1)withtrans-cyclooctenes(TCO)asthedienophile,thefastestkineticseverreportedforanybioorthogonalreaction.Inaddition,inverse-Electron-DemandDiels-Alderreactionoftetrazinestrans-cycloocteneaformsastablecovalentbondanddoesnotrequireCu-catalystorelevatedtemperatures.Inapplicationssuchasinvivocancerimagingorpre‐targetedcelllabelingstudieswhererapidreactionkineticsaredesired,afasterhydrogensubstitutedCy®3Tetrazineprobewouldbeaprobeofchoice.
Cy®3isabright,water-soluble,andpHinsensitiveorange-fluorescentdyethatcanbeexcitedusingthe532nmor555nmlaserlineandvisualizedwithTRITC(tetramethylrhodamine)filtersets.Cy3conjugatesgivelessbackgroundthanTAMRAandmostothercommonlyusedfluorescentdyes.
Cy®DyeisatrademarkofGEHealthcare.
| Abs/EmMaxima | 550/570nm |
| Extinctioncoefficient | 150,000 |
| Flowcytometrylaserline | 532or555nm |
| Microscopylaserline | 532or555nm |
| Spectrallysimilardyes | AlexaFluor®555,Atto™555,CF™555Dye,DyLight™555 |
| Molecularweight | 894.05 |
| CAS | N/A |
| Solubility | Water,DMSO,DMF |
| Purity | >90%(HPLC) |
| Appearance | Redsolid |
| Storageconditions | -20C |
| Shippingconditions | Ambienttemperature |
